生物信息学 - "global name 'start' is not defined"?

Bioinformatics - "global name 'start' is not defined"?

设计并编写一个可以检测跨膜结构域的程序,称为 TMscanner.py。该程序应调用您编写的另外两个函数:getProteinRegion 应该 return 十个氨基酸 window(最初获取氨基酸 1-10,下一次获取 2-20,依此类推) TMscanner 还应该重复调用第二个函数 testForTM,它应该计算和 return 这十个非极性氨基酸的小数部分。非极性氨基酸是 [A,V,L,I,P,M,F,W]

示例:

protein = 'AVLIPMFWGSTNQYDEKRH' #Only the first 9 are nonpolar
results = tmScanner(protein)
print "Results are", results

它应该打印出来:

Results are [.8, .7, .6, .5, .4, .3, .2, .1, 0, 0]

-- #测试代码

def getProteinRegion(protein, start):
#return first 10 aa in protein
    return protein[start:start+10]

def testForTM(protein):
#return ratio of nonpolar aa from a sample of first 10; move them up one step and repeat
    nonpolar = 0
    for aa in getProteinRegion(protein, start):
        if aa in ['A', 'V', 'L', 'I', 'P', 'M', 'F', 'W']:
            nonpolar = nonpolar + 1.0
    return nonpolar / 10.0


def tmSCANNER(protein):
#while the tested in testForTM is less than 10, append testforTM function to results; print results
    results = []
    start = 0
    while start <= 10:
        results = results.append(testForTM(protein))
        return results
        start = start + 1

##TESTING

#print getProteinRegion('AVLIPMFWGSTCNQYDEKRH')
#print testForTM('AVLIPMFW')
print tmSCANNER('AVLIPMFWGSTCNQYDEKRH')

--

Traceback (most recent call last):
  File "TMscanner.py", line 29, in <module>
    print tmSCANNER('AVLIPMFWGSTCNQYDEKRH')
  File "TMscanner.py", line 21, in tmSCANNER
    results = results.append(testForTM(protein))
  File "TMscanner.py", line 10, in testForTM
    for aa in getProteinRegion(protein, start):
NameError: global name 'start' is not defined

您的函数 testForTM 没有使用 start 参数,没有声明名为 start 的局部变量,但它调用了 getProteinRegion(protein, start)。因此,Python 假定您必须使用全局变量——但您也没有具有该名称的全局变量。所以它引发了一个异常。

您可能想要添加 start 作为参数:

def testForTM(protein, start):

然后,在 tmSCANNER 中的调用代码中,您 do 有一个名为 start 的局部变量,这可能是您想要的值作为 start 参数传递:

results = results.append(testForTM(protein, start))