只有我的一些物种被转换为 NCBI ID,使用 biopython 将物种转换为 ID

Only some of my species are being converted to NCBI IDs, using biopython to convert species to ID

我有一些代码可以从带有下划线的列表中去除物种名称,并将其转换为适合 NCBI 的格式,然后搜索与该物种名称关联的 ID,但是由于某种原因,这不起作用我的输入文件中的每个条目。我已附上我的代码、输入文件的子集和输出文件的子集。

from Bio import Entrez
import time


Entrez.email = 'fake.email@isp.com'

def get_tax_id(species):
    species = species.replace('_', '+').strip()
    search = Entrez.esearch(term=species, db='taxonomy', retmode='xml')
    record = Entrez.read(search)
    return record['IdList']

current_time = time.strftime("%d.%m.%y %H:%M", time.localtime())

output_name = 'test#%s.txt' % current_time

file = open(output_name, "w+")

listoforganisms = [x.split('\t')[0] for x in open("OGTlist.csv").readlines()]

if __name__ == '__main__':
    organisms = listoforganisms
    for organism in organisms:
        taxid = get_tax_id(organism)
        stringid = str(taxid)
        strippedid = stringid.strip("'[]'")
        if len(stringid) <= 2:
            file.write('\n' + str(organism) + ',ERROR_no_ID_match')
        else:
            file.write('\n' + str(organism) + ',' + str(strippedid))

所以这段代码打印一个结果文件,如果转换成功,打印物种名称和 ID,如果不成功,它只是打印一个错误,我的结果文件如下所示:

micromonospora_inyonensis,47866
viola_arvensis,97415
amycolatopsis_albidoflavus,102226
tetragenococcus_koreensis,290335
panaeolus_papilionaceus,330517
geomys_pinetis,100306
vibrio_lutjanus,ERROR_no_ID_match
succiniclasticum_ruminis,40841
microtetraspora_malaysiensis,161358
blarina_carolinensis,183658
amycolatopsis_palatopharyngis,187982
rhodosporidium_toruloides,5286
geobacter_bemidjiensis,225194
acinetobacter_haemolyticus,29430
actinoplanes_tereljensis,571912
phyllostomus_hastatus,9423
phacidium_infestans,66518
dorea_formicigenerans,39486
hoeflea_marina,274592
naemacyclus_minor,64355
methanosaeta_thermophila,2224
pholiota_carbonaria,227966
sphingomonas_faeni,185950
helicobacter_pullorum,35818
solitalea_koreensis,543615
dermacoccus_profundi,322602
pseudomonas_pictorum,86184
actinomadura_livida,79909
leptonycteris_curasoae,55054
psychrobacter_salsus,219741
vibrio_inusitatus,413402
stereum_rameale,ERROR_no_ID_match
photorhabdus_temperata,574560
clitocybe_lignatilis,5634
actinocorallia_glomerata,46203
aspergillus_giganteus,5060
erwinia_amylovora,552
hydrogenoanaerobacterium_saccharovorans,474960
mycobacterium_aichiense,1799
nocardia_pneumoniae,228601
bacillus_pocheonensis,363869
streptomonospora_alba,183763
exobasidium_gracile,190086
phenylobacterium_zucineum,284016
amsonia_tabernaemontana,144544
rattus_fuscipes,10119
jannaschia_rubra,282197
hereroa_rehneltiana,ERROR_no_ID_match

我从中获取物种名称的文件如下所示:

micromonospora_inyonensis   28  DSMZ
viola_arvensis  23  DSMZ
amycolatopsis_albidoflavus  28  DSMZ
tetragenococcus_koreensis   28  DSMZ
panaeolus_papilionaceus 24  DSMZ
geomys_pinetis  36.3    white
vibrio_lutjanus 30  DSMZ
succiniclasticum_ruminis    37  DSMZ
microtetraspora_malaysiensis    28  DSMZ
blarina_carolinensis    36.8    white
amycolatopsis_palatopharyngis   28  DSMZ
rhodosporidium_toruloides   23  DSMZ
geobacter_bemidjiensis  30  DSMZ
acinetobacter_haemolyticus  28  DSMZ
actinoplanes_tereljensis    28  DSMZ
phyllostomus_hastatus   34.7    white
phacidium_infestans 25  DSMZ
dorea_formicigenerans   37  DSMZ
hoeflea_marina  28  DSMZ
naemacyclus_minor   22  DSMZ
methanosaeta_thermophila    58.3333333333   DSMZ
pholiota_carbonaria 25  DSMZ
sphingomonas_faeni  22  DSMZ
helicobacter_pullorum   37  DSMZ
solitalea_koreensis 28  DSMZ
dermacoccus_profundi    28  DSMZ
pseudomonas_pictorum    28  DSMZ
actinomadura_livida 28  DSMZ
leptonycteris_curasoae  35.7    white
psychrobacter_salsus    22  DSMZ
vibrio_inusitatus   28  DSMZ
stereum_rameale 20  DSMZ
photorhabdus_temperata  28.6666666667   DSMZ
clitocybe_lignatilis    25  DSMZ
actinocorallia_glomerata    28  DSMZ
aspergillus_giganteus   24.5    DSMZ
erwinia_amylovora   26.6666666667   DSMZ
hydrogenoanaerobacterium_saccharovorans 37  DSMZ
mycobacterium_aichiense 37  DSMZ
nocardia_pneumoniae 28  DSMZ
bacillus_pocheonensis   30  DSMZ
streptomonospora_alba   28  DSMZ
exobasidium_gracile 20  DSMZ
phenylobacterium_zucineum   30  DSMZ
amsonia_tabernaemontana 23  DSMZ
rattus_fuscipes 37.5    white
jannaschia_rubra    25  DSMZ
hereroa_rehneltiana 23  DSMZ

我的实际输入文件大约有 2000 个条目,答案是物种名称不正确还是 NCBI 上不存在所有物种的 ID 一样简单,有没有人有解决方案来以编程方式解决这个问题?

第一个答案是物种名称不存在。你可以在 ncbi 网站上查看。像这儿: https://www.ncbi.nlm.nih.gov/search/?term=Stereum+rameale

https://www.ncbi.nlm.nih.gov/search/?term=vibrio_lutjanus

如果您查看其他网站,无论如何,Vibrio lutjanus 似乎都不存在。例如 https://www.arb-silva.de/search/

没有解决这个问题的方法(如果找到分类单元 ID),但您可以仔细检查名称是否正确。分类学很困难,每个人都有不同的名字,而且有很多同义词。您可以使用 api 的分类名称网站,例如 gbif 或全球名称。

[编辑]

如果物种不可用,您还可以检查属的分类单元 ID。这里可以下载NCBI的分类信息:

ftp://ftp.ncbi.nlm.nih.gov/pub/taxonomy/new_taxdump/

您需要下载 zip 文件,可能还需要文件 rankedlineage.dmp 和 merged.dmp 全球名称网站也可用于属级。不知道来自 BioPython 的 entrez 是否可以查找属级别的 id,也许这也是一个选项。