从 v.0.11.6 开始,如何为 fastQC 启用 kmer 图

How to enable kmer plot for fastQC up from v. 0.11.6

我 运行 fastQC 在 Linux 机器上使用命令行。

在 v.0.11.5 之前,我还习惯将 kmer 图作为输出。

现在,从 v.0.11.6 开始,默认情况下禁用 Kmer 输出(请参阅发行说明),我找不到再次启用它的方法。

我试过 运行 这个程序,例如使用 --kmers=7 选项,但在 .zip 输出中和绘图中都没有 km​​er 估计。

它是完全弃用了还是我遗漏了手册中的内容?

提前致谢。

勾选https://github.com/s-andrews/FastQC/releases

RELEASE NOTES FOR FastQC v0.11.6

This update fixes some bugs and updates some of the functionality to accommodate changes in some of the sequencing platforms.

There is one major change which is that by default we now disable the kmer module. With the inclusion of the adapter plot the value of the information in the Kmer plot is often not great, and it is easy to confound it if there are any over-represented sequences, or primer compositional bias. Overall therefore we consider it best to not routinely include this module.

If you want to turn this module back on, then simply edit the limits.txt file in the Configuration folder of the FastQC installation and change the line near the top which says:

kmer ignore 1

..to..

kmer ignore 0

..and the module will be re-enabled.